ELISA, Flow Cytometry (FACS), Western Blotting (WB)
Spezifität
Reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD as demonstrated by ELISA and flow cytometry.
Produktmerkmale
Source: Pooled antisera from goats hyperimmunized with normal human IgM and IgG, and human IgM, IgG and IgA paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Aufreinigung
Affinity chromatography on pooled human immunoglobulin covalently linked to agarose.
Working Dilution: <= 1 µg/10^6 cells or 1:5,000-1:20,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid cycles of freezing and thawing.
Lagerung
4 °C
Fan, Longnecker: "The Ig-like v-type domain of paired Ig-like type 2 receptor alpha is critical for herpes simplex virus type 1-mediated membrane fusion." in: Journal of virology, Vol. 84, Issue 17, pp. 8664-72, (2010) (PubMed).