ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Spezifität
Reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry.
Produktmerkmale
Source: Pooled antisera from goats hyperimmunized with mouse IgM paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Aufreinigung
Affinity chromatography on pooled IgG covalently linked to agarose.
Working Dilution: 1:4,000-1:8,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C
Margry, Kersemakers, Hoek, Arkesteijn, Wieland, van Eden, Broere: "Activated peritoneal cavity B-1a cells possess regulatory B cell properties." in: PLoS ONE, Vol. 9, Issue 2, pp. e88869, (2014) (PubMed).