ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Spezifität
Reacts with the heavy and light chains of goat IgG as demonstrated by ELISA.
Produktmerkmale
Source: Pooled antisera from swine hyperimmunized with goat IgG. To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA for conformance to characteristics of a standard reference reagent.
Aufreinigung
Affinity chromatography on human IgG covalently linked to agarose.
Working Dilution: 1:4,000-1:8,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C
Kitevska, Roberts, Pantaki-Eimany, Boyd, Scott, Hawkins: "Analysis of the minimal specificity of caspase-2 and identification of Ac-VDTTD-AFC as a caspase-2-selective peptide substrate." in: Bioscience reports, (2014) (PubMed).