ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
Spezifität
Reacts with the heavy chain of mouse IgG 1 as demonstrated by ELISA and flow cytometry.
Produktmerkmale
Source: Pooled antisera from goats hyperimmunized with mouse IgG 1 paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry to conform to characteristics of a standard reference reagent.
Working Dilution: <= 1 µg/10^6 cells Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2.
Konservierungsmittel
Azide free
Handhabung
Each reagent is stable for the period shown on the bottle label if stored as directed.
Lagerung
4 °C
Harada, Tanaka, Motomura, Harada, Ohno, Ohno, Yanagi, Inoue, Kubo: "The 3' Enhancer CNS2 Is a Critical Regulator of Interleukin-4-Mediated Humoral Immunity in Follicular Helper T Cells." in: Immunity, Vol. 36, Issue 2, pp. 188-200, (2012) (PubMed).