Applications: FC - Quality tested ELISA - Quality tested FLISA - Quality tested IHC-FS - Reported in literature , ELISPOT - Reported in literature
Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, CY5, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:100 - 1:400 PE, CY5, and AF647 conjugates 1 g/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added, store at 2-8 °C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/Sodium azide
Konservierungsmittel
Without preservative
Handhabung
Each reagent is stable for the period shown on the bottle label if stored as directed.