The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Produktmerkmale
Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal cat serum in agar block titration.
Aufreinigung
Delipidated, heat inactivated lyophilized stable whole serum No adsorption required.
Immunogen
Purified normal IgG isolated from pooled cat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal cat serum, to prepare an immunoadsorbent for the production of Ig-free cat serum, plasma or non-Ig preparations.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is reconstituted by adding 1 mL sterile distilled water.
Buffer
No foreign proteins added.
Konservierungsmittel
Without preservative
Handhabung
Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.