Ziege anti-Hund IgM (Fc Region) Antikörper - Preadsorbed

Produktnummer ABIN457680

Produktdetails

Target: IgM

Bindungsspezifität: Fc Region

Reaktivität: Hund

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Spezifität: The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity.

Produktmerkmale: Antibody titre: Precipitin titre not less than 1:32 when tested against pooled normal dog serum in agar-block immunodiffusion titration.

Aufreinigung: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Purified IgM isolated from dog serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:3,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Konzentration: 10 mg/mL

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody