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Maus anti-Human IgG1 (Fc Region) Antikörper (FITC)

ELISA, WB Monoclonal NI 132 (HP 6186) IgG1 kappa FITC

Produktnummer ABIN457886

Datenblatt (PDF)

Target Alle IgG1 Produkte

IgG1
Bindungsspezifität
65

25

9

6

5

3

3

2

2

1

1

1

1

1
Fc Region
Reaktivität
105

25

23

9

7

5

4

3

3

2

1

1

1
Human
Wirt
62

42

41

23

10

6

1
Maus
Klonalität
94

70
Monoklonal
Konjugat
51

22

21

18

11

8

7

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
FITC
Applikation
93

57

57

45

31

20

18

15

11

10

10

9

6

5

3

2

2

2

1
ELISA, Western Blotting (WB)

Spezifität

The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig.

Produktmerkmale

Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG1, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 2.7.

Aufreinigung

Purified

Immunogen

Highly purified monoclonal IgG1 isolated from human serum.

Klon

NI 132 (HP 6186)

Isotyp

IgG1 kappa
Mouse anti-Human IgG1 (Hinge Region) antibody
ELISA Monoclonal 4E3 IgG1 unconjugated

Mouse anti-Human IgG1 (Hinge Region) antibody (HRP)
ELISA Monoclonal 4E3 IgG1 HRP

Mouse anti-Human IgG1 (Hinge Region) antibody (FITC)
ELISA Monoclonal 4E3 IgG1 FITC

Mouse anti-Human IgG1 (Hinge Region) antibody (PE)
ELISA Monoclonal 4E3 IgG1 PE

Mouse anti-Human IgG1 (Fc Region) antibody
ELISA, FLISA Monoclonal HP6001 IgG2b unconjugated

Mouse anti-Human IgG1 (Hinge Region) antibody (Alkaline Phosphatase (AP))
ELISA Monoclonal 4E3 IgG1 Alkaline Phosphatase (AP)

Mouse anti-Human IgG1 (Fc Region) antibody (Alkaline Phosphatase (AP))
ELISA, FLISA Monoclonal HP6001 IgG2b Alkaline Phosphatase (AP)

Mouse anti-Human IgG1 (Hinge Region) antibody (Biotin)
ELISA Monoclonal 4E3 IgG1 Biotin

Mouse anti-Human IgG1 (Fc Region) antibody (HRP)
ELISA, FLISA Monoclonal HP6001 IgG2b HRP

Rabbit anti-Human IgG1 (Internal Region) antibody
ELISA, WB, IHC Polyclonal IgG unconjugated

Applikationshinweise

To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Kommentare

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Konzentration

0.4 mg/mL

Buffer

Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel

Without preservative

Handhabung

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
Target

IgG1

Abstract

IgG1 Produkte

Substanzklasse

Antibody