The activity of the antiserum is directed to human IgG4 subclass. Precipitating polyclonal sheep antisera to human IgG4, subclass specific Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: The antigenic differences between subclasses are considerably less pronounced than those between classes or betw
Aufreinigung
Delipidated, heat inactivated lyophilized stable whole serum Immunoaffinity adsorption with insolubilized highly purified myeloma proteins of the other subclass or other serum proteins as required.
Immunogen
Purified homogenous IgG4 is isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify and measure the concentration of IgG4 subclass in serum or other body fluids. Human IgG constitutes about 75 % of the total Immunoglobulins in the serum of normal adults. It consists of four subclasses which are distinguished in isotypic heterogeneity of the gamma chains, and in physicochemical, biological and effector properties. The term
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is reconstituted by adding 1 mL sterile distilled water.
Buffer
No foreign proteins added.
Konservierungsmittel
Without preservative
Handhabung
Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.