The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion
Produktmerkmale
Precipitating polyclonal rabbit antiserum to monkey Fab of IgG Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal monkey serum in agar block titration.
Aufreinigung
Delipidated, heat inactivated lyophilized stable whole serum Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other subunits of the immunoglobulin or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.
Immunogen
Purified Fab of normal Rhesus monkey IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
In immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is reconstituted by adding 1 mL sterile distilled water.
Buffer
No foreign proteins added.
Konservierungsmittel
Without preservative
Handhabung
Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.