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Ziege anti-Maus IgG2 Antikörper (TRITC) - Preadsorbed

IHC, ICC, IF Polyclonal IgG TRITC
Produktnummer ABIN458405
  • Target Alle IgG2 Produkte
    IgG2
    Reaktivität
    • 22
    • 12
    • 7
    • 7
    • 6
    • 1
    • 1
    Maus
    Wirt
    • 20
    • 10
    • 10
    • 10
    • 6
    Ziege
    Klonalität
    • 30
    • 26
    Polyklonal
    Konjugat
    • 21
    • 9
    • 8
    • 8
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    TRITC
    Applikation
    • 30
    • 28
    • 24
    • 20
    • 14
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Spezifität
    This renders it specific for use in test systems containing material of human origin (e. g. human tissue/mouse monoclonal antibody to a human tissue constituent/anti mouse Ig isotype-specific immunoconjugate.
    Aufreinigung
    Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
    Immunogen
    Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a and Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotyp
    IgG
  • Applikationshinweise
    In immunocytochemical and immunohistochemical single and double staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, as indicating second antibody conjugate to analyze or separate human cells after reacting with monoclonal mouse antibodies in immunofluorescence microscopy, fluorescence activated cell sorting and flow cytometry, to identify and measure IgG2 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted.
    The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    Konzentration
    10 mg/mL
    Buffer
    TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    Konservierungsmittel
    Without preservative
    Handhabung
    Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgG2
    Abstract
    IgG2 Produkte
    Substanzklasse
    Antibody
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