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Ziege anti-Maus IgG2 Antikörper - Preadsorbed

ELISA, IHC, ICC Polyclonal IgG unconjugated
Produktnummer ABIN458515
  • Target Alle IgG2 Produkte
    IgG2
    Reaktivität
    • 22
    • 12
    • 7
    • 7
    • 6
    • 1
    • 1
    Maus
    Wirt
    • 20
    • 10
    • 10
    • 10
    • 6
    Ziege
    Klonalität
    • 30
    • 26
    Polyklonal
    Applikation
    • 29
    • 28
    • 24
    • 20
    • 15
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Spezifität
    The reactivity of the antiserum is directed to the subclass IgG2a and IgG2b as tested against mouse sera and a panel of purified homogenous immunoglobulins.
    Aufreinigung
    Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
    Immunogen
    Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotyp
    IgG
  • Applikationshinweise
    As unlabelled primary or secondary reagent for indirect detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10.000.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    Konzentration
    10 mg/mL
    Buffer
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    Konservierungsmittel
    Without preservative
    Handhabung
    Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgG2
    Abstract
    IgG2 Produkte
    Substanzklasse
    Antibody
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