Kaninchen anti-Maus IgG2 Antikörper (FITC)

Produktnummer ABIN458530

Produktdetails

Target: IgG2

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Immunofluorescence (IF)

Spezifität: The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b as tested against mouse sera and a panel of purified homogenous immunoglobulins.

Produktmerkmale: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to mouse IgG2, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) is approximately 2.0.

Aufreinigung: Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: To identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Konzentration: 10 mg/mL

Buffer: Fluorochrome-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgG2

Abstract: IgG2 Produkte

Substanzklasse: Antibody