Ziege anti-Human IgA Antikörper (Biotin)

Produktnummer ABIN5608053

Produktdetails

Target: IgA

Fragment: F(ab')2 fragment

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Verwendungszweck: F(ab')2 Human IgA (alpha chain) Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.

Produktmerkmale: F(ab')2 Anti-Human IgM Fc5μ Alkaline Phosphatase Antibody generated in goat detects specifically the Fc5μ portion of the human IgM heavy chain.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Human IgA alpha heavy chain

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Human IgA Biotin Antibody is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:200 - 1:1,000 Immunohistochemistry Dilution: 1:500 - 1:5,000 Western Blot Dilution: 1:20,000 - 1:400,000 ELISA Dilution: 1:20,000 - 1:400,000 IF Microscopy Dilution: 1:200 - 1:1,000 Other: 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Konzentration: 1.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgA

Abstract: IgA Produkte

Substanzklasse: Antibody

Hintergrund: F(ab')2 Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.