Ziege anti-Human IgA (Heavy Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN5608053

Produktdetails

Target: IgA

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy Chain

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Produktmerkmale: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Human IgA alpha heavy chain

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:5,000

Application Note: F(ab')2 Anti-Human IgA Biotin Antibody is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:20,000 - 1:400,000

Flow Cytometry Dilution: 1:200 - 1:1,000

Western Blot Dilution: 1:2,000 - 1:10,000

Other Performance Data: This product has been overfilled to ensure total recovery of stated quantity.

Kommentare:

This product has been overfilled to ensure total recovery of stated quantity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgA

Abstract: IgA Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat F(ab')2 anti-Human IgA (alpha chain) Antibody biotin Conjugation, Goat F(ab')2 anti-Human IgA alpha biotin Conjugated Antibody

Background: F(ab')2 Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.