Ziege anti-Human lambda (Light Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN5608136

Produktdetails

Target: lambda

Bindungsspezifität: Light Chain

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Human

Produktmerkmale: Anti-Human lambda chain peroxidase conjugated antibody generated in goat detects specifically human lambda chain. This secondary peroxidase conjugated antibody anti-Human lambda chain is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Human lambda chain was produced by repeated immunization with human lambda light chain in goat.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: This secondary peroxidase conjugated antibody anti-Human lambda chain is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Human lambda chain Antibody peroxidase conjugated has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:16,000 to 1:64,000 is suggested for this product.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:1,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: lambda

Andere Bezeichnung: lambda )

Hintergrund:

Synonyms: goat anti-Human λ (lambda chain) Antibody peroxidase Conjugated, goat anti-Human lambda chain HRP Conjugated Antibody

Background: The anti-Human λ (lambda chain) Antibody detects the lambda chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG lambda is conjugated to peroxidase.