Esel anti-Human IgM (Fc5mu Region) Antikörper (PE) - Preadsorbed

Produktnummer ABIN5608181

Produktdetails

Target: IgM

Fragment: F(ab')2 fragment

Bindungsspezifität: Fc5mu Region

Reaktivität: Human

Wirt: Esel

Klonalität: Polyklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Human IgM Fc5mu  fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.

Haltbarkeit: 6 months

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Donkey F(ab')2 anti-Human IgM Fc5u Antibody Phycoerythrin Conjugation, Donkey F(ab')2 anti-Human IgM Fc5u PE Conjugated Antibody

Background: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody generated in donkey detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.