Ziege anti-Human IgA (Free & Bound), (Secretory Component) Antikörper (FITC)

Produktnummer ABIN568606

Produktdetails

Target: IgA

Bindungsspezifität: Free & Bound, Secretory Component

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktmerkmale: Molar Ratio: 3.0 moles TRITC per mol of Chicken IgG

Aufreinigung: Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and purification of the IgG fraction by DEAE-Chromatography.

Immunogen: Purified free Human Secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Absorption / Emission: 550 nm / 570 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1.0 mL of sterile distilled water

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Handhabung: If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.

Antigendetails

Target: IgA

Abstract: IgA Produkte

Substanzklasse: Antibody

Hintergrund: Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM.Synonyms: Human Immunoglobulin A