Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Western Blotting (WB)
Produktmerkmale
Molar Ratio: Peroxidase/IgG aprox. 1.0
Aufreinigung
Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
Immunogen
Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 1,0 mL distilled water. Working dilutions should be prepared by adding sterile PBS, pH 7.2
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -28 °C for longer.