Esel anti-Ziege IgG Antikörper (Cy3) - Preadsorbed

Produktnummer ABIN6698843

Hersteller Produkt- Nr.: 605-704-125

Produktdetails

Target: IgG

Reaktivität: Ziege

Wirt: Esel

Klonalität: Polyklonal

Konjugat: Cy3

Applikation: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 605-704-125

Hersteller: Rockland

Verwendungszweck: Goat IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins

Produktmerkmale: donkey anti-Goat IgG CY3 Conjugated Pre-Adsorbed Antibody,Anti-Goat IgG Cy3 Antibody generated in donkey detects goat IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Goat IgG whole molecule

Isotyp: IgG

Labeling Ratio: 8.1

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Kommentare:

Goat IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed antibody has been tested by dot blot and western blot. These antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Suggested Applications: IHC, Multiplex, Other

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Masuda-Murakami, Kobayashi, Wang, Yamada, Kimura, Hossain, Matsumoto: "Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells." in: Acta histochemica, Vol. 112, Issue 5, pp. 452-8, (2011) (PubMed).

Jain, Liu, Ramani, Arauz, Ishitsuka, Ragunathan, Park, Chen, Xiang, Ha: "Probing cellular protein complexes using single-molecule pull-down." in: Nature, Vol. 473, Issue 7348, pp. 484-8, (2011) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.