Ziege anti-Human IgM Antikörper (DyLight 549)

Produktnummer ABIN6698960

Hersteller Produkt- Nr.: 609-142-007

Produktdetails

Target: IgM

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 549

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 609-142-007

Hersteller: Rockland

Verwendungszweck: Human IgM (mu chain) Antibody DyLight™ 549 Conjugated

Produktmerkmale: Goat Anti-Human IgM (mu chain) Antibody DyLight™ 549 Conjugated, Goat Anti Human IgM (mu chain) Antibody DyLight™ 549 Conjugated,Anti-Human IgM (mu heavy chain) DyLight 549 generated in goat detects specifically Human IgM mu heavy chain.

Immunogen: Human IgM whole molecule

Isotyp: IgG

Labeling Ratio: 3.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Anti-Human IgM (mu heavy chain) DyLight 549 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: Microarray, WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Fathi, Dahlke, Krähling, Kupke, Okba, Raadsen, Heidepriem, Müller, Paris, Lassen, Klüver, Volz, Koch, Ly, Friedrich, Fux, Tscherne, Kalodimou, Schmiedel, Corman, Hesterkamp, Drosten, Loeffler, Haagmans, Sutter, Becker, Addo: "Increased neutralization and IgG epitope identification after MVA-MERS-S booster vaccination against Middle East respiratory syndrome." in: Nature communications, Vol. 13, Issue 1, pp. 4182, (2022) (PubMed).

Paris, Heidepriem, Tsouka, Liu, Mattes, Pinzón Martín, Dallabernardina, Mende, Lindner, Wawrzinek, Rademacher, Seeberger, Breitling, Bischoff, Wolf, Loeffler: "Automated Laser-Transfer Synthesis of High-Density Microarrays for Infectious Disease Screening." in: Advanced materials (Deerfield Beach, Fla.), Vol. 34, Issue 23, pp. e2200359, (2022) (PubMed).

Wallménius, Påhlson, Nilsson: "Retrospective serological study of Rickettsia spp. and Borrelia spp. antibodies in patients with peripheral facial nerve palsy." in: Infection ecology & epidemiology, Vol. 11, Issue 1, pp. 1987058, (2021) (PubMed).

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.