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Ziege anti-Human IgM Antikörper (DyLight 649)

Rockland 609-143-007 WB, FLISA, FM Polyclonal IgG DyLight 649
Rockland
Produktnummer ABIN6698961
Hersteller Produkt- Nr.: 609-143-007
  • Target Alle IgM Produkte
    IgM
    Reaktivität
    • 225
    • 162
    • 91
    • 42
    • 34
    • 34
    • 29
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Human
    Wirt
    • 372
    • 231
    • 87
    • 22
    • 14
    • 5
    • 4
    • 1
    • 1
    Ziege
    Klonalität
    • 539
    • 96
    Polyklonal
    Konjugat
    • 122
    • 86
    • 86
    • 66
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    DyLight 649
    Applikation
    • 385
    • 289
    • 283
    • 242
    • 182
    • 137
    • 91
    • 91
    • 63
    • 61
    • 54
    • 53
    • 45
    • 30
    • 18
    • 17
    • 10
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Hersteller Produkt- Nr.
    609-143-007
    Hersteller
    Rockland
    Verwendungszweck
    Human IgM (mu chain) Antibody DyLight™ 649 Conjugated
    Produktmerkmale
    Goat Anti-Human IgM (mu chain) Antibody DyLight™ 649 Conjugated, Goat Anti Human IgM (mu chain) Antibody DyLight™ 649 Conjugated,Anti-Human IgM (mu heavy chain) DyLight 649 generated in goat detects specifically Human IgM mu heavy chain.
    Immunogen
    Human IgM whole molecule
    Isotyp
    IgG
    Labeling Ratio
    1.8
  • Applikationshinweise

    FLISA_Dilution: >1:20,000

    IF_Microscopy_Dilution: >1:5,000

    Western_Blot_Dilution: >1:10,000

    Other: User Optimized

    Kommentare

    The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Anti-Human IgM (mu heavy chain) DyLight 649 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Ishida, Corrigan, Chen, Liu, Kim, Song, Rutledge, Magee, LaBaer, Lowary, Lin, Achkar: "Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates." in: EBioMedicine, Vol. 99, pp. 104897, (2024) (PubMed).

  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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