Kaninchen anti-Affe IgG Antikörper (DyLight 680) - Preadsorbed

Produktnummer ABIN6698968

Produktdetails

Target: IgG

Reaktivität: Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: DyLight 680

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: rabbit anti-Monkey IgG DyLight™ 680 conjugated antibody, rabbit anti-Monkey IgG antibody DyLight™ 680 conjugation

Background: Conjugated Anti-Monkey IgG (H&L) DyLight™ 680 Conjugated antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This DyLight™680 conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform immunofluorescence, flow cytometry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Aufreinigung: Preadsorption: immunoaffinity chromatography using Monkey IgG coupled to agarose beads

Immunogen:

Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 2.8

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Monkey IgG (H&L) DyLight™ 680 conjugated Antibody is suitable for use in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity and signal detection should be optimized by the end user.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody