Ziege anti-Affe IgM Antikörper (DyLight 800)

Produktnummer ABIN6698970

Produktdetails

Target: IgM

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), FLISA, Fluorescence Microscopy (FM)

Verwendungszweck: Monkey IgM (mu chain) Antibody DyLight™800 Conjugated

Produktmerkmale: Goat Anti-Monkey IgM Antibody DyLight™ 800 Conjugation, Goat Anti-Monkey IgM DyLight™800 Conjugated Antibody,Anti-Monkey IgM (mu chain) antibody generated in goat detects specifically monkey IgM.

Immunogen: Monkey IgM whole molecule

Isotyp: IgG

Labeling Ratio: 2.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Kommentare:

Anti-Monkey IgM (mu chain) DyLight™800 has been tested by dot blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Specific conditions for reactivity and signal detection should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.