Kaninchen anti-Maus IgG Antikörper (DyLight 800)

Produktnummer ABIN6699036

Hersteller Produkt- Nr.: 610-445-002

Kurzübersicht für Kaninchen anti-Maus IgG Antikörper (DyLight 800) (ABIN6699036)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Kaninchen

Hersteller Produkt- Nr.: 610-445-002

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated

Produktmerkmale: rabbit anti-Mouse IgG Antibody DyLight™ 800 conjugation, rabbit anti-Mouse IgG DyLight™800 conjugated Antibody,Anti-Mouse IgG DyLight 800 Antibody generated in rabbit detects reactivity to Mouse IgG.

Immunogen: Mouse IgG, whole molecule

Labeling Ratio: 2.5

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Mouse IgG DyLight 800 Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Conjugated Anti-Mouse IgG is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight conjugated antibodies are effective alternatives to Alexa 800 and IR Dye 800 conjugates. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Balic, Albargy, Luu, Kirby, Jayasekara, Mansell, Garama, De Nardo, Baschuk, Louis, Humphries, Fitzgerald, Latz, Gough, Mansell: "STAT3 serine phosphorylation is required for TLR4 metabolic reprogramming and IL-1β expression." in: Nature communications, Vol. 11, Issue 1, pp. 3816, (2020) (PubMed).

Zhang, Li, Chen, Chen, Mi, Ma, Wang, Sun, Liu, Cui: "Non-genomic mechanisms mediate androgen-induced PSD95 expression." in: Aging, Vol. 11, Issue 8, pp. 2281-2294, (2020) (PubMed).

Wang, Sheng, He, Chen, Li, Tan, Wang, Lan: "A simple and highly purified method for isolation of glomeruli from the mouse kidney." in: American journal of physiology. Renal physiology, Vol. 317, Issue 5, pp. F1217-F1223, (2020) (PubMed).

Mitra, Bodor, David, Abdul-Zani, Mata, Neumann, Reither, Tischer, Jansen: "Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin." in: Nature communications, Vol. 11, Issue 1, pp. 501, (2020) (PubMed).

Zhang, Kang, Zhou, Cui, Jia, Hu, Ji, Yuan, Cui, Shi: "Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling." in: Scientific reports, Vol. 8, Issue 1, pp. 10726, (2018) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.