Ziege anti-Maus IgG Antikörper (DyLight 800) - Preadsorbed

Produktnummer ABIN6699041

Hersteller Produkt- Nr.: 610-145-121

Produktdetails

Target: IgG

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 610-145-121

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Produktmerkmale: Goat Anti-Mouse IgG Secondary Antibody DyLight™800 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™800 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™800 conjugated secondary antibody,Anti-Mouse IgG DyLight 800 Antibody generated in goat detects reactivity to Mouse IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Isotyp: IgG

Labeling Ratio: 2.25

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Mouse IgG DyLight 800 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

García-Poyatos, Arora, Calvo, Marques, Kirschke, Galardi-Castilla, Lembke, Meer, Fernández-Montes, Ernst, Haberthür, Hlushchuk, Vázquez, Vermathen, Enríquez, Mercader: "Cox7a1 controls skeletal muscle physiology and heart regeneration through complex IV dimerization." in: Developmental cell, Vol. 59, Issue 14, pp. 1824-1841.e10, (2024) (PubMed).

Cantacorps, Zhu, Yagoub, Coull, Falck, Chesters, Ritter, Serrano-Lope, Tscherepentschuk, Kasch, Paterson, Täger, Baidoe-Ansah, Pandey, Igual-Gil, Braune, Lippert: "Developmental metformin exposure does not rescue physiological impairments derived from early exposure to altered maternal metabolic state in offspring mice." in: Molecular metabolism, Vol. 79, pp. 101860, (2024) (PubMed).

Zhou, Zhang, Wang, Yang, Jiao, Lu, Li, Jiang, Zhang: "Targeting of HBP1/TIMP3 axis as a novel strategy against breast cancer." in: Pharmacological research, Vol. 194, pp. 106846, (2023) (PubMed).

Yang, Zhou, Zhang, Wang, Wang, Cheng, Li, Jiang, Yang, Zhang: "The transcription factor HBP1 promotes ferroptosis in tumor cells by regulating the UHRF1-CDO1 axis." in: PLoS biology, Vol. 21, Issue 7, pp. e3001862, (2023) (PubMed).

Tapia-Galisteo, Sánchez Rodríguez, Aguilar-Sopeña, Harwood, Narbona, Ferreras Gutierrez, Navarro, Martín-García, Corbacho, Compte, Lacadena, Blanco, Chames, Roda-Navarro, Álvarez-Vallina, Sanz: "Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer." in: Oncoimmunology, Vol. 11, Issue 1, pp. 2034355, (2022) (PubMed).

Zhang, Fu, Sun, Zou, Qiu, Zhang, Su, Shi, Li, Xu: "Pharmacological suppression of Nedd4-2 rescues the reduction of Kv11.1 channels in pathological cardiac hypertrophy." in: Frontiers in pharmacology, Vol. 13, pp. 942769, (2022) (PubMed).

Cao, Cheng, Wang, Liu, Yang, Jiang, Li, Zhang: "HBP1-mediated transcriptional repression of AFP inhibits hepatoma progression." in: Journal of experimental & clinical cancer research : CR, Vol. 40, Issue 1, pp. 118, (2021) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Xu, Yu, Ma, Li, Shen, Chen, Huang, Zhang, Deng, Wang: "IL-6 promotes nuclear translocation of HIF-1α to aggravate chemoresistance of ovarian cancer cells." in: European journal of pharmacology, Vol. 894, pp. 173817, (2021) (PubMed).

García-Poyatos, Cogliati, Calvo, Hernansanz-Agustín, Lagarrigue, Magni, Botos, Langa, Amati, Vázquez, Mercader, Enríquez: "Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish." in: EMBO reports, Vol. 21, Issue 7, pp. e50287, (2021) (PubMed).

Liu, Wang, Yang, Cheng, Zhou, Li, Jiang, Zhang: "GP73-mediated secretion of AFP and GP73 promotes proliferation and metastasis of hepatocellular carcinoma cells." in: Oncogenesis, Vol. 10, Issue 10, pp. 69, (2021) (PubMed).

Acín-Pérez, Hernansanz-Agustín, Enríquez: "Analyzing electron transport chain supercomplexes." in: Methods in cell biology, Vol. 155, pp. 181-197, (2020) (PubMed).

Xue, Cao, Cheng, Wang, Liu, Yang, Li, Jiang, Li, Zhao, Zhang: "Acetylation of alpha-fetoprotein promotes hepatocellular carcinoma progression." in: Cancer letters, Vol. 471, pp. 12-26, (2020) (PubMed).

Guan, Zhou, Hafner, Blake, Chalouni, Chen, De Bruyn, Giltnane, Hartman, Heidersbach, Houtman, Ingalla, Kategaya, Kleinheinz, Li, Martin, Modrusan, Nannini, Oeh, Ubhayakar, Wang, Wertz, Young, Yu, Sampath, Hager, Friedman, Daemen, Metcalfe: "Therapeutic Ligands Antagonize Estrogen Receptor Function by Impairing Its Mobility." in: Cell, Vol. 178, Issue 4, pp. 949-963.e18, (2020) (PubMed).

Takahashi, Ranjan, Chen, Suzuki, Shibata, Hirose, Hirose, Sasaki, Abe, Chen, He, Zhang, Takigawa, Tsukiyama, Watanabe, Fujii, Iida, Yamamoto, Yamaguchi, Suzuki, Matsumoto, Nakayama, Washburn, Saraf, Florens, Sato, Tomomori-Sato, Conaway, Conaway, Hatakeya: "The role of Mediator and Little Elongation Complex in transcription termination." in: Nature communications, Vol. 11, Issue 1, pp. 1063, (2020) (PubMed).

Mitra, Bodor, David, Abdul-Zani, Mata, Neumann, Reither, Tischer, Jansen: "Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin." in: Nature communications, Vol. 11, Issue 1, pp. 501, (2020) (PubMed).

Asthana, Stern, Tang, Bugga, Li, Ferguson, Asthana, Bao, Drezek: "Development of a Novel Class of Self-Assembling dsRNA Cancer Therapeutics: A Proof-of-Concept Investigation." in: Molecular therapy oncolytics, Vol. 18, pp. 419-431, (2020) (PubMed).

Owusu-Brackett, Zhao, Akcakanat, Evans, Yuca, Dumbrava, Janku, Meric-Bernstam: "Targeting PI3Kβ alone and in combination with chemotherapy or immunotherapy in tumors with PTEN loss." in: Oncotarget, Vol. 11, Issue 11, pp. 969-981, (2020) (PubMed).

Hosawi, Humphries, Coward, Knight, Humphries, Lennon: "Global proteomic analysis of insulin receptor interactors in glomerular podocytes." in: Wellcome open research, Vol. 5, pp. 202, (2020) (PubMed).

Kennedy, Rinker, Broadie: "Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model." in: BMC biology, Vol. 18, Issue 1, pp. 94, (2020) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.