Ziege anti-Maus IgG (Fc Region) Antikörper (DyLight 800)

Produktnummer ABIN6699050

Hersteller Produkt- Nr.: 610-145-003

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 610-145-003

Hersteller: Rockland

Verwendungszweck: Mouse IgG Fc Antibody DyLight™ 800 Conjugated

Produktmerkmale: Goat Anti Mouse IgG F(c) Antibody DyLight™ 800 Conjugated, Goat Anti-Mouse IgG Fc Antibody DyLight™ 800 Conjugated, Goat Anti Mouse IgG Fc Fragment Antibody DyLight™ 800 Conjugated,Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Immunogen: Mouse IgG F(c) fragment

Isotyp: IgG

Labeling Ratio: 2.3

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Mouse IgG F(c) DyLight 800 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Szewczyk, Günther, Japtok, Frech, Naumann, Lee, Hermann: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." in: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).

Greenstein, Ng, Barrales, Tan, Braun, Al-Sady: "Local chromatin context regulates the genetic requirements of the heterochromatin spreading reaction." in: PLoS genetics, Vol. 18, Issue 5, pp. e1010201, (2022) (PubMed).

White, Milad, Sellers, Bernatchez: "Effect of Dysferlin Deficiency on Atherosclerosis and Plasma Lipoprotein Composition Under Normal and Hyperlipidemic Conditions." in: Frontiers in physiology, Vol. 12, pp. 675322, (2021) (PubMed).

Wang, Zhu, Shan, Wang, Yin, Yang, Wang, Zhang, Wang, Liang, Zhang, Jiang, Dong, Jiang, Qi, Feng: "Spy1, a unique cell cycle regulator, alters viability in ALS motor neurons and cell lines in response to mutant SOD1-induced DNA damage." in: DNA repair, Vol. 74, pp. 51-62, (2019) (PubMed).

Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.