Kaninchen anti-Maus IgG1 (Heavy Chain) Antikörper (DyLight 649)

Produktnummer ABIN6699057

Hersteller Produkt- Nr.: 610-443-040

Produktdetails

Target: IgG1

Bindungsspezifität: Heavy Chain

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: DyLight 649

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 610-443-040

Hersteller: Rockland

Verwendungszweck: Mouse IgG1 (Gamma 1 chain) Antibody DyLight™ 649 Conjugated

Produktmerkmale: Rabbit Anti Mouse IgG1 (Gamma 1 chain) Antibody DyLight 649™ Conjugated, Rabbit Anti-Mouse IgG1 Antibody DyLight 649™ Conjugated,Anti-Mouse IgG1 DyLight 649 Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain).

Immunogen: Mouse IgG1 heavy chain

Isotyp: IgG

Labeling Ratio: 3.2

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: Microarray

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

White, Couetil, Richard, Marti, Wilson: "Microarray molecular mapping of horses with severe asthma." in: Journal of veterinary internal medicine, Vol. 38, Issue 1, pp. 477-484, (2024) (PubMed).

Wyler, Sage, Marti, White, Gerber: "Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma." in: Journal of veterinary internal medicine, Vol. 37, Issue 1, pp. 328-337, (2023) (PubMed).

Birras, White, Jonsdottir, Novotny, Ziegler, Wilson, Frey, Torsteinsdottir, Alcocer, Marti: "First clinical expression of equine insect bite hypersensitivity is associated with co-sensitization to multiple Culicoides allergens." in: PloS one, Vol. 16, Issue 11, pp. e0257819, (2021) (PubMed).

Novotny, White, Wilson, Stefánsdóttir, Tijhaar, Jonsdóttir, Frey, Reiche, Rose, Rhyner, Schüpbach-Regula, Torsteinsdóttir, Alcocer, Marti: "Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity." in: Allergy, Vol. 76, Issue 4, pp. 1147-1157, (2021) (PubMed).

White, Moore-Colyer, Marti, Hannant, Gerber, Coüetil, Richard, Alcocer: "Antigen array for serological diagnosis and novel allergen identification in severe equine asthma." in: Scientific reports, Vol. 9, Issue 1, pp. 15170, (2020) (PubMed).

White, Moore-Colyer, Marti, Coüetil, Hannant, Richard, Alcocer: "Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma." in: Journal of veterinary internal medicine, Vol. 33, Issue 5, pp. 2327-2335, (2020) (PubMed).

Antigendetails

Target: IgG1

Abstract: IgG1 Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.