Esel anti-Kaninchen IgG Antikörper (DyLight 649) - Preadsorbed

Produktnummer ABIN6699111

Hersteller Produkt- Nr.: 611-743-127

Produktdetails

Target: IgG

Reaktivität: Kaninchen

Wirt: Esel

Klonalität: Polyklonal

Konjugat: DyLight 649

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 611-743-127

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Produktmerkmale: Donkey Anti-Rabbit IgG Antibody DyLight 649™ Conjugated, Donkey Anti Rabbit IgG DyLight 649™ Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 649 Antibody generated in donkey detects reactivity to Rabbit IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Isotyp: IgG

Labeling Ratio: 3.1

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Rabbit IgG (H&L) DyLight 649 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IHC, Multiplex

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Ayyar, Ettayebi, Salmen, Karandikar, Neill, Tenge, Crawford, Bieberich, Prasad, Atmar, Estes: "CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection." in: Nature communications, Vol. 14, Issue 1, pp. 1148, (2023) (PubMed).

Onoda, Hagiwara, Kubota, Yanagita, Takeda, Umezawa: "A Novel Staining Method for Detection of Brain Perivascular Injuries Induced by Nanoparticle: Periodic Acid-Schiff and Immunohistochemical Double-Staining." in: Frontiers in toxicology, Vol. 4, pp. 825984, (2022) (PubMed).

Onoda, Kawasaki, Tsukiyama, Takeda, Umezawa: "Carbon nanoparticles induce endoplasmic reticulum stress around blood vessels with accumulation of misfolded proteins in the developing brain of offspring." in: Scientific reports, Vol. 10, Issue 1, pp. 10028, (2020) (PubMed).

Krzisch, Fülling, Jabinet, Armida, Gebara, Cassé, Habbas, Volterra, Hornung, Toni: "Synaptic Adhesion Molecules Regulate the Integration of New Granule Neurons in the Postnatal Mouse Hippocampus and their Impact on Spatial Memory." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 27, Issue 8, pp. 4048-4059, (2018) (PubMed).

Lee, Nevo: "Microvascular endothelial cells from preeclamptic women exhibit altered expression of angiogenic and vasopressor factors." in: American journal of physiology. Heart and circulatory physiology, Vol. 310, Issue 11, pp. H1834-41, (2017) (PubMed).

Tanase, Yokoo, Matsumura, Kinoshita, Kikuchi, Suemori, Ohyama: "Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm." in: Biochemical and biophysical research communications, Vol. 482, Issue 4, pp. 764-770, (2017) (PubMed).

Onoda, Takeda, Umezawa: "Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle." in: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 4, (2017) (PubMed).

Onoda, Kawasaki, Tsukiyama, Takeda, Umezawa: "Perivascular Accumulation of β-Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles." in: Frontiers in cellular neuroscience, Vol. 11, pp. 92, (2017) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.