Ziege anti-Kaninchen IgG Antikörper (DyLight 800) - Preadsorbed

Produktnummer ABIN6699120

Produktdetails

Target: IgG

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody

Background: Conjugated DyLight™ 800 secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Rabbit IgG whole molecule

Isotyp: IgG

Labeling Ratio: 2.5

Anwendungsinformationen

Applikationshinweise:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Pereira, Bacman, Arguello, Zekonyte, Williams, Edgell, Moraes: "mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels." in: EMBO molecular medicine, Vol. 10, Issue 9, (2018) (PubMed).

Nguyen, Williams, Young, Ma, Li, Levis, Brown, Small: "FLT3 activating mutations display differential sensitivity to multiple tyrosine kinase inhibitors." in: Oncotarget, Vol. 8, Issue 7, pp. 10931-10944, (2017) (PubMed).

Meyer, Neeley, Baker, Washabaugh, Flesher, Nelson, Frankel, Lumeng, Lyssiotis, Wynn, Rhim, ORourke: "Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer." in: Biochemistry and biophysics reports, Vol. 7, pp. 144-149, (2016) (PubMed).

Klammer, Kaminski, Zedler, Oppermann, Blencke, Marx, Müller, Tebbe, Godl, Schaab: "Phosphosignature predicts dasatinib response in non-small cell lung cancer." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 9, pp. 651-68, (2013) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody