Ziege anti-Kaninchen IgG Antikörper (DyLight 488)

Produktnummer ABIN6699123

Produktdetails

Target: IgG

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 488

Applikation: Western Blotting (WB), Immunofluorescence (IF), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB), Multiplex Assay (MA)

Verwendungszweck: Rabbit IgG (H&L) Secondary Antibody DyLight™ 488 Conjugated

Produktmerkmale: Goat anti-Rabbit IgG Antibody DyLight™488 Conjugation, Goat anti-Rabbit IgG DyLight™ 488 Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 488 Antibody generated in goat detects reactivity to Rabbit IgG.

Aufreinigung: Conjugated Anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.

Immunogen: Rabbit IgG whole molecule

Isotyp: IgG

Labeling Ratio: 3.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Rabbit IgG (H&L) DyLight 488 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: FC, IHC, WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Dufour, Gillet, Frankel, Weibel, Emonet: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.