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Ziege anti-Ratte IgG Antikörper (Cy3) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Cy3
Produktnummer ABIN6699132
  • Target Alle IgG Produkte
    IgG
    Reaktivität
    • 724
    • 702
    • 604
    • 482
    • 403
    • 243
    • 212
    • 176
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 84
    • 59
    • 48
    • 35
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Wirt
    • 1791
    • 1576
    • 574
    • 244
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Ziege
    Klonalität
    • 3450
    • 178
    • 1
    Polyklonal
    Konjugat
    • 589
    • 418
    • 417
    • 383
    • 282
    • 276
    • 244
    • 119
    • 108
    • 67
    • 66
    • 64
    • 61
    • 58
    • 52
    • 50
    • 48
    • 46
    • 45
    • 44
    • 41
    • 39
    • 36
    • 33
    • 33
    • 31
    • 30
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    Cy3
    Applikation
    • 1956
    • 1852
    • 1810
    • 1522
    • 933
    • 909
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 54
    • 53
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Produktmerkmale

    Synonyms: Goat Anti Rat IgG CY3 Conjugated Antibody, Goat Anti-Rat IgG Antibody CY3 Conjugation

    Background: Anti-Rat IgG (H&L) CY3 Antibody generated in goat detects reactivity to Rat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to CY3.

    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Rat IgG whole molecule

    Isotyp
    IgG
    Labeling Ratio
    9.8
  • Applikationshinweise

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Torigoe, Yamauchi, Zhu, Kobayashi, Murakami: "Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium." in: Scientific reports, Vol. 5, pp. 12258, (2016) (PubMed).

  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
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