Ziege anti-Ratte IgG Antikörper (DyLight 800)
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- Target Alle IgG Produkte
- IgG
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Reaktivität
- Ratte
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- DyLight 800
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Applikation
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Hersteller Produkt- Nr.
- 612-145-002
- Hersteller
- Rockland
- Verwendungszweck
- Rat IgG (H&L) Antibody Dylight™ 800 Conjugated
- Produktmerkmale
- Goat Anti-Rat IgG DyLight 800™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 800™ Conjugation,
- Aufreinigung
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. This antibody will react with heavy chains of Rat IgG and with light chains of most Rat immunoglobulins.
- Immunogen
- Rat IgG, whole molecule
- Isotyp
- IgG
- Labeling Ratio
- 2.0
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- Applikationshinweise
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- Kommentare
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Rat IgG DyLight™ 800 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
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