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Rekombinanter Alpaca anti-Maus IgG2 Antikörper (Atto 488)

WB, IHC, ICC, IF, FACS Monoclonal 14A4 Recombinant Antibody Atto 488 single-domain Antibody (sdAb)
Produktnummer ABIN7272926
  • Target Alle IgG2 Produkte
    IgG2
    Antikörpertyp
    Recombinant Antibody
    Fragment
    single-domain Antibody (sdAb)
    Reaktivität
    • 22
    • 12
    • 7
    • 7
    • 6
    • 1
    • 1
    Maus
    Wirt
    • 20
    • 11
    • 10
    • 10
    • 5
    Alpaca
    Expressionssystem
    E.coli
    Klonalität
    • 31
    • 25
    Monoklonal
    Konjugat
    • 21
    • 9
    • 8
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Atto 488
    Applikation
    • 30
    • 27
    • 24
    • 20
    • 14
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Spezifität
    Mouse IgG2, does not cross-react with rabbit, guinea pig or chicken immunoglobulins
    Aufreinigung
    Produced in: E.coli
    Immunogen
    FluoTag-X2 anti-Mouse IgG2 is a species-specific FluoTag directed against IgG2a and IgG2b subtype from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications. Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
    Klon
    14A4
    Labeling Ratio
    Two site-specifically conjugated fluorophores per sdAb.
  • Applikationshinweise
    1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
    Buffer
    The single sdAb clone was lyophilized from PBS pH 7.4.
    Lagerung
    -20 °C/-80 °C
    Informationen zur Lagerung
    Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
  • Target
    IgG2
    Abstract
    IgG2 Produkte
    Substanzklasse
    Antibody
    Hintergrund
    In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
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