ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)
Hersteller Produkt- Nr.
610-1302-1.0
Hersteller
Rockland
Verwendungszweck
Goat Anti-Mouse IgG H&L (HRP)
Spezifität
IgG (H&L)
Aufreinigung
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.
Güteklasse
High-quality product from Rockland
Immunogen
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat. Immunogen Type: Native Protein
Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse HRP antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Restore with deionized water (or equivalent)
Konzentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungsmittel
Gentamicin sulfate
Vorsichtsmaßnahmen
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Haltbarkeit
12 months
Kücük, Abbey, Schmitt, Henninger, Fritz: "Cardiomyocytes, cardiac endothelial cells and fibroblasts contribute to anthracycline-induced cardiac injury through RAS-homologous small GTPases RAC1 and CDC42." in: Pharmacological research, Vol. 203, pp. 107165, (2024) (PubMed).
Hummels, Berry, Li, Taguchi, Min, Walker, Marks, Bernhardt: "Coordination of bacterial cell wall and outer membrane biosynthesis." in: Nature, Vol. 615, Issue 7951, pp. 300-304, (2023) (PubMed).
Halmi, Wu, Taneyhill: "Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly." in: F1000Research, Vol. 11, pp. 741, (2023) (PubMed).
Castro-Córdova, Otto-Medina, Montes-Bravo, Brito-Silva, Lacy, Paredes-Sabja: "Redistribution of the Novel Clostridioides difficile Spore Adherence Receptor E-Cadherin by TcdA and TcdB Increases Spore Binding to Adherens Junctions." in: Infection and immunity, Vol. 91, Issue 1, pp. e0047622, (2023) (PubMed).
Bina, Hines, Sanyal, Taneyhill: "Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches." in: Journal of developmental biology, Vol. 11, Issue 1, (2023) (PubMed).
Thakkar, Pradhan, Duva, Carreno, Sahu, Thiruthuvanathan, Campbell, Gallego, Bhagat, Rivera, Choudhary, Olea, Sabalza, Shapiro, Lee, Quinn, Mantzaris, Chu, Will, Pirofski, Krammer, Verma, Halmos: "Study of efficacy and longevity of immune response to third and fourth doses of COVID-19 vaccines in patients with cancer: A single arm clinical trial." in: eLife, Vol. 12, (2023) (PubMed).
Montero, Garcia-Betancourt, Vidal, Velasco, Palacios, Schneider, Vega, Gómez, Montecinos, Soto-Shara, Oñate, Carreño: "A chimeric protein-based vaccine elicits a strong IgG antibody response and confers partial protection against Shiga toxin-producing Escherichia coli in mice." in: Frontiers in immunology, Vol. 14, pp. 1186368, (2023) (PubMed).
Kim, Oh, Kim, Shin, Lee, Kim: "β‑carotene attenuates muscle wasting in cancer cachexia by regulating myogenesis and muscle atrophy." in: Oncology reports, Vol. 51, Issue 1, (2023) (PubMed).
Liu, Samant, Vasa, Pedrigi, Oguz, Ryu, Wei, Anderson, Agrawal, Chatzizisis: "Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis." in: PloS one, Vol. 18, Issue 1, pp. e0280385, (2023) (PubMed).
Strohmeier, Amanat, Campbell, Traquina, Coffman, Krammer: "A CpG 1018 adjuvanted neuraminidase vaccine provides robust protection from influenza virus challenge in mice." in: NPJ vaccines, Vol. 7, Issue 1, pp. 81, (2022) (PubMed).
Hormazabal, Saavedra, Espinoza-Arratia, Martinez, Cruces, Alfaro, Loyola: "Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality control." in: Nucleic acids research, Vol. 50, Issue 4, pp. 1875-1887, (2022) (PubMed).
Ortega, Lobos-González, Reyna-Jeldes, Cerda, De la Fuente-Ortega, Castro, Bernal, Coddou: "Corrigendum to "The Ω-3 fatty acid docosahexaenoic acid selectively induces apoptosis in tumor-derived cells and suppress tumor growth in gastric cancer" [Eur. J. Pharmacol. (2021) 173910]." in: European journal of pharmacology, Vol. 906, pp. 174287, (2021) (PubMed).
Carreño, Alshammary, Singh, Raskin, Amanat, Amoako, Gonzalez-Reiche, van de Guchte, Study Group, Srivastava, Sordillo, Sather, van Bakel, Krammer, Simon: "Evidence for retained spike-binding and neutralizing activity against emerging SARS-CoV-2 variants in serum of COVID-19 mRNA vaccine recipients." in: EBioMedicine, Vol. 73, pp. 103626, (2021) (PubMed).
Arunkumar, Bhavsar, Li, Strohmeier, Chromikova, Amanat, Bunyatov, Wilson, Ellebedy, Boons, Simon, de Vries, Krammer: "Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus." in: Nature communications, Vol. 12, Issue 1, pp. 6161, (2021) (PubMed).
Ortega, Lobos-González, Reyna-Jeldes, Cerda, De la Fuente-Ortega, Castro, Bernal, Coddou: "The Ω-3 fatty acid docosahexaenoic acid selectively induces apoptosis in tumor-derived cells and suppress tumor growth in gastric cancer." in: European journal of pharmacology, Vol. 896, pp. 173910, (2021) (PubMed).
Ramirez, Aonbangkhen, Wu, Naftaly, Tang, OMeara, Woo: "Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells." in: ACS chemical biology, Vol. 15, Issue 4, pp. 1059-1066, (2021) (PubMed).
Yang, He, Zhang, Lu, Zhang, Ren, Tian, Yang, Chen, Li, Fu, Allain, Li, Wang: "Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp." in: Frontiers in cellular and infection microbiology, Vol. 10, pp. 145, (2021) (PubMed).
Fegan, Calmettes, Islam, Ahn, Chaudhuri, Yu, Gray-Owen, Moraes, Schryvers: "Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species." in: Frontiers in immunology, Vol. 10, pp. 247, (2020) (PubMed).
Henninger, Pohlmann, Ziegler, Ohlig, Schmitt, Fritz: "Distinct contribution of Rac1 expression in cardiomyocytes to anthracycline-induced cardiac injury." in: Biochemical pharmacology, Vol. 164, pp. 82-93, (2020) (PubMed).
Noppes, Müller, Bennien, Holtemeyer, Palatini, Leidolf, Alber, Geyer: "Homo- and heterodimerization is a common feature of the solute carrier family SLC10 members." in: Biological chemistry, Vol. 400, Issue 10, pp. 1371-1384, (2020) (PubMed).
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1