Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)
Kreuzreaktivität
Affe
Aufreinigung
DEAE-column Chromatography
Immunogen
Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 1 mL sterile distilled water
Konzentration
10 mg/mL
Buffer
PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.