Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.
Aufreinigung
This product is a F(ab')2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.
ELISA, IHC, WB, ICC, IA
Polyclonal
Alkaline Phosphatase (AP)
Applikationshinweise
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
Konzentration
10.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungsmittel
Gentamicin sulfate
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.