Ziege anti-Kaninchen IgG (F(ab')2 Region) Antikörper

Produktnummer ABIN964861

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: F(ab')2 Region

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum.

Aufreinigung: This product is a F(ab')2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum.  No reaction was observed against Rabbit IgG F(c), anti-Goat IgG F(c) or anti-Pepsin.

Immunogen:

Immunogen: Rabbit IgG F(ab')2 fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

ELISA Dilution: 1:20,000-1:100,000

Western Blot Dilution: 1:2,000-1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Handhabung: Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat F(ab')2 Anti-RABBIT IgG F(ab')2 fragment Antibody, goat F(ab')2 Anti-RABBIT IgG Fab2 fragment Antibody, goat Fab2 Anti Rabbit IgG Fab2

Background: F(ab')2 Anti-Rabbit IgG F(ab')2 Antibody generated in goat detects Rabbit F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.