Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN964977

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Verwendungszweck: Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Spezifität: IgG (H&L)

Produktmerkmale: Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen:

Immunogen: Rabbit IgG whole molecule

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. ELISA: 1:10,000-1:100,000 IHC: 1:500-1:5,000 WB: 1:5,000-1:40,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Cong, Gong, Yang, Xia, Zhang: "miR-22 Suppresses Tumor Invasion and Metastasis in Colorectal Cancer by Targeting NLRP3." in: Cancer management and research, Vol. 12, pp. 5419-5429, (2020) (PubMed).

Nuwer, Fleck: "Anterograde trafficking signals in GABAA subunits are required for functional expression." in: Channels (Austin, Tex.), Vol. 13, Issue 1, pp. 440-454, (2020) (PubMed).

Agrawal, Lehtonen, Uusi-Mäkelä, Jain, Viitala, Määttä, Kähkönen, Azizi, Riihimäki, Kulomaa, Johnson, Hytönen, Airenne: "Molecular features of steroid-binding antidins and their use for assaying serum progesterone." in: PLoS ONE, Vol. 14, Issue 2, pp. e0212339, (2019) (PubMed).

Shrestha, Ahn, Staples, Sathyan, Karpova, Foltz, Basrai: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." in: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).

Millonig, Ganzleben, Peccerella, Casanovas, Brodziak-Jarosz, Breitkopf-Heinlein, Dick, Seitz, Muckenthaler, Mueller: "Sustained submicromolar H2O2 levels induce hepcidin via signal transducer and activator of transcription 3 (STAT3)." in: The Journal of biological chemistry, Vol. 287, Issue 44, pp. 37472-82, (2013) (PubMed).

Grando, Bystryn, Chernyavsky, Frusić-Zlotkin, Gniadecki, Lotti, Milner, Pittelkow, Pincelli: "Apoptolysis: a novel mechanism of skin blistering in pemphigus vulgaris linking the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis." in: Experimental dermatology, Vol. 18, Issue 9, pp. 764-70, (2009) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Synonyms: Rabbit Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.