Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (FITC) - Preadsorbed

Produktnummer ABIN965162

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: FITC

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Spezifität: F(ab')2 Mouse IgG (H&L) Antibody Fluorescein ConjugatedAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.

Produktmerkmale: F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotyp: IgG

Labeling Ratio: 2.52

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

ELISA Dilution: 1:20,000 - 1:100,000

Flow Cytometry Dilution: 1:500 - 1:2,500

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat F(ab')2 Anti-Mouse IgG Antibody Fluorescein Conjugation, Goat Fab2 Anti-Mouse IgG FITC conjugated Antibody

Background: F(ab')2 Anti-Mouse IgG (H&L) Fluorescein Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.