Esel anti-Maus IgG (Heavy & Light Chain) Antikörper (TRITC) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
- Fragment
- F(ab')2 fragment
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Maus
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Wirt
- Esel
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Klonalität
- Polyklonal
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Konjugat
- TRITC
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
- Produktmerkmale
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Aufreinigung
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG whole molecule
- Isotyp
- IgG
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- Applikationshinweise
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Handhabung
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light. - Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Donkey F(ab')2 Anti-Mouse IgG Antibody rhodamine Conjugation, Donkey Fab2 Anti-Mouse IgG TRITC Conjugated Antibody
Background: F(ab')2 Anti-Mouse IgG (H&L) Rhodamine Antibody generated in donkey was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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