Kaninchen anti-Huhn IgG (Heavy & Light Chain) Antikörper (FITC) - Preadsorbed

Produktnummer ABIN965308

Produktdetails

Target: IgG

Fragment: Fab fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum.

Produktmerkmale: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Chicken IgG whole molecule

Isotyp: IgG

Labeling Ratio: 2.1

Anwendungsinformationen

Applikationshinweise:

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Rabbit Fab Anti-Chicken Antibody Fluorescein Conjugation, Rabbit Fab Anti-Chicken FITC Conjugated Antibody

Background: Fab Anti-Chicken IgG Fluorescein Antibody generated in rabbit detects chicken IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.