Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN965359

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.

Produktmerkmale: Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen: Anti-Mouse IgG (H&L) was produced by repeated immunization with mouse IgG whole molecule in goat.
Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Mouse IgG (H&L) peroxidase conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Referenzen

Goodpaster, Randolph-Habecker: "A flexible mouse-on-mouse immunohistochemical staining technique adaptable to biotin-free reagents, immunofluorescence, and multiple antibody staining." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 62, Issue 3, pp. 197-204, (2014) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Synonyms: Mouse IgG (H&L) Antibody, Gt-a-Mouse F(ab')2 Peroxidase conjugated, Mouse F(ab')2 Peroxidase Antibody in goat, Goat F(ab')2 peroxidase conjugated Secondary Antibody.