ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
Spezifität
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
Produktmerkmale
Anti-Rabbit IgG whole molecule antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Rabbit IgG whole molecule antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Aufreinigung
Preadsorption: Solid phase absorption
Immunogen
Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat. Immunogen Type: Native Protein
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
Konzentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungsmittel
Gentamicin sulfate
Handhabung
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Lagerung
4 °C
Informationen zur Lagerung
Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
Haltbarkeit
12 months
Lu, Stump, Seavey: "Novel method of monitoring trace cytokines and activated STAT molecules in the paws of arthritic mice using multiplex bead technology." in: BMC immunology, Vol. 11, pp. 55, (2011) (PubMed).